home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Shareware Overload Trio 2
/
Shareware Overload Trio Volume 2 (Chestnut CD-ROM).ISO
/
dir26
/
med9410l.zip
/
M94A1928.TXT
< prev
next >
Wrap
Text File
|
1994-10-24
|
2KB
|
37 lines
Document 1928
DOCN M94A1928
TI A naturally occurring single amino acid substitution within the V3
region of HIV-1 ENV protein affects the viral cellular host range and
antigenic structure.
DT 9412
AU Shioda T; Oka S; Ida S; Nokihara K; Toriyoshi H; Mori S; Takebe Y;
Kimura S; Shimada K; Nagai Y; Institute of Medical Science, Univ. of
Tokyo, Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):42 (abstract no. 143A). Unique
Identifier : AIDSLINE ICA10/94370647
AB OBJECTIVE: To examine sequence changes in the V3 domain of gp120 during
the course of HIV-1 infection and to learn their significance for the
viral cellular host range and antigenic drift. METHODS: Sera
sequentially obtained from an infected individual were subjected to
reverse-transcription of the V3 region followed by PCR and cloning by
the TA cloning kit. Multiple clones thus obtained at each time point
were sequenced. Recombinant viruses carrying different V3 sequences were
generated and tested for their in vitro cellular tropism. Synthetic
peptides mimicking the V3 sequence were used for ELISA as antigens.
RESULTS: All the clones obtained at the clinical phase I had aspartic
acid (D) at position 323 in the V3, but this residue was replaced with
lysine (K) in about one-third of the clones at the phase IV. This single
amino acid change led to broadening cell tropism in vitro and resulted
in different recognition by several of anti HIV human sera. CONCLUSIONS:
V3 domain may evolve in vivo to increase net charge to broaden in vitro
cell tropism and to drift antigenically in the disease course. This
notion appear to be worthy of assessing with more clinical cases.
DE *Amino Acid Sequence Antigenic Variation Cloning, Molecular Human
HIV Antigens/*ULTRASTRUCTURE HIV Envelope Protein gp120/*GENETICS
HIV-1/*GENETICS Polymerase Chain Reaction Viral Envelope
Proteins/*ULTRASTRUCTURE MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).